| A proven single step method
for the isolation and purification of total RNA from Tissues/Cells/Bacteria/Plant/Yeast
and biological fluids.
This method is based on the principle of a patent pending formulation
of a 14M solution of guanidine salts and urea (Chaosolv) which act
as denaturing agents and on the use of a specific RNA Binding
Resin to purify total RNA. Bio/RNA-XCell™
purifies the RNA by mixing the aqueous phase with RNA Binding
Resin. The resin binds only the total RNA while other impurities
are washed with 75% ethanol. The pure RNA is eluted from the
resin with water or buffer. RNA thus prepared is free from
impurities such as traces of guanidine salts, phenol, etc. which
might interfere in subsequent reactions.
Bio/RNA-XCell™ is highly reliable and produces reproducible
and reliable results in 30-45 minutes!
Applications
The total RNA isolated by this method is undegraded and free of
proteins and DNA contamination. It can be used for Northern
analysis, dot blot hybridization, poly A+ selection, in vitro translation,
RNase protection assay, molecular cloning, and for reverse transcriptase/polymerase
chain reaction (PCR*). The simplicity of the RNA isolation using
this system makes it possible to process simultaneously a large
number of samples. This method can also isolate total RNA from
very small biological samples (biopsies, etc).
Reagents Supplied
Reagents Required but not supplied
Chloroform, isopropanol and 75% ethanol. (These chemicals
should be of high quality, minimum ACS grade); DEPC treated water.
Stability
Bio/RNA-XCell™ is stable for more than nine
months when stored in the dark at 2-8°C.
Yield and Purity of RNA
The final preparation of undegraded RNA is free of DNA and proteins
and has an A260/A280 ratio of approximately 1.8-2.0.
| Yield for 10 million mammalian
cells |
100-200µg |
| Yield for 100mg tissue |
150-500µg (depending on tissue) |
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Special Handling Precautions
Bio/RNA-XCell™ contains poison (phenol)
and irritant (guanidine salts). CAN BE FATAL. Use
gloves and eye protection (shield, safety goggles). Do not
get in skin or clothing. Avoid breathing vapour. Read warning
note on the bottle. For laboratory use only.
IN CASE OF CONTACT: IMMEDIATELY FLUSH EYES OR SKIN WITH
A LARGE AMOUNT OF SALINE OR WATER FOR AT LEAST 15 MINUTES AND SEEK
IMMEDIATE MEDICAL ATTENTION.
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