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Parallab 350™ -
Fully Automated System Operation
The Parallab 350™ is designed for true unattended automation
and walk away processing. The system stores all of the components
for the reactions and cleanup (e.g. template, Big Dye® cocktail,
magnetic beads for purification, alcohol and water) for a full day's
run on a chilled work deck. During the reaction assembly, the robot
will move to the required plate location and remove the insulated
lid to provide access for the Nano-Pipetter. The Nano-Pipetter will
aspirate a small volume of reagent into each of its 96 thin-walled
glass tubes, each serving as a reaction vessel. After all of the
components are automatically drawn into the Nano-Pipetter, they
will be effectively mixed using a high speed shuttling of the fluids
inside of the tubes. The Nano-Pipetter will then be placed into
the onboard high speed thermal cycler, where the 96 tubes will be
reversibly sealed and thermal cycled. Following thermal cycling,
magnetic bead solution is aspirated into each reaction tube where
the purification takes place. The 96 cleaned up final products are
then dispensed into an output plate for off-line analysis. The Nano-Pipetter
is then automatically cleaned with the onboard wash station in preparation
for the next run.
Parallab 350™ System Components:
- Heavy duty gantry style robotic arm
- Automated 96-channel Nano-Pipetter
- Onboard high-speed air thermal cycler
- Onboard automated wash station for the Nano-Pipetter
- Onboard purification station; including magnetic seperator
tool and agitating trough for bead solution
- Eighteen chilled reagent nests with insulated lids for
96 or 384 microwell plates and reagent troughs
- Water station for in-line laboratory grade water
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System control PC with a 17" monitor
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Vacuum pump, transfer pump, waste holding
tank and associated tubing
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Completely enclosed workdeck
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PCR decontamination components (OPTIONAL)
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