Parallab 350™ - Nano-Pipettor Washing Station and Decontamination Station

The Parallab 350™ includes an onboard wash station to effectively clean the Nano-Pipetter between runs. This clean-in-place capability eliminates the need and cost of disposable tips often required in other systems. After each run the Nano-Pipetter is automatically docked into the wash station where the entire device is cleaned using high pressure water and air jets. Each of the 96 glass tubes, their plungers and tips are thoroughly cleaned on the inside and outside during this process.

Washes and dries the Nano-Pipetter for reuse: Glass tubes Inside Outside Plunger tips

For systems running PCR, which may require additional assurances against carry-over, the Parallab incorporates a proprietary breakthrough decontamination technology in addition to the standard wash station. This process effectively decontaminates all 96 glass tubes to eliminate carry-over without inhibiting further reactions. In an experiment shown below, a single glass tube was used for 20 PCR amplification runs. These runs were segmented into 10 ‘rounds’ of amplification. Each round consisted of:

1. Run a 35 cycle PCR amplification
2. Run an agarose gel analysis of sample (+)
3. Run the proprietary wash and decontamination protocol
4. Run a 35 cycle amplification with no template to test for carry-over (Round 10 is 45 cycles)
5. Run an agarose gel analysis of sample (-)

Conclusion: Zero carry-over and no inhibition of subsequent PCR** amplifications

Agarose gel analysis of repeated PCR reactions showing zero carry-over and no subsequent reaction inhibition even after over-cycling. L = 100bp ladder. 5ul total reaction size.