1. SpeedUp Sequencing

DNA Walking SpeedUp™ Premix Kit provides a simple and fast PCR-based method using our patented DW-ACP primer system for amplifying unknown sequences adjacent to known genomic DNA or cDNA sequences and providing templates for direct sequencing of the amplified unknown target DNA sequence. Whole genomic DNA or single-strand cDNA generated by RT can be used as a template for direct amplification and sequencing or cloning of an unknown target sequence. The beauty of the DNA Walking SpeedUp™ Premix Kit comes from the ACP's maximised specificity along with the optimised two-stage PCR conditions.

Features

• No complex enzyme process
• No library construction
• No library screening
• No cloning for sequencing
• No false products

2. SpeedUp BAC Clone Sequencing

DNA Walking SpeedUp™ Premix Kit provides a simple and fast PCR-based method using our patented DW-ACP primer system for amplifying unknown sequences adjacent to known sequences (or BAC vector sequence) and providing templates for direct sequencing of the amplified unknown target DNA sequence. Without shotgun cloning of BAC clone, BAC clone DNA can be used as a template for direct sequencing of the insert DNA.

Features

• Simple and fast method
• 1-2 kb direct sequencing
• Small amount of starting material required
• Cost-effective
• No false products

3. SpeedUp Genome Walking

DNA Walking SpeedUp™ Premix Kit provides a simple and fast PCR-based method using our patented DW-ACP primer system for amplifying unknown sequences adjacent to known genomic DNA sequences and providing templates for direct sequencing of the amplified unknown target DNA sequence. This kit can be used for a variety of applications such as analysis of gene structure (exon/intron junction), or direct amplification and sequencing, or cloning of unknown genomic DNA sequences.

Features

• No complex enzyme process
• No library construction
• No library screening
• No cloning for sequencing
• No false products

4. SpeedUp Transgene Location Detection

DNA Walking SpeedUp™ Premix Kit provides a simple and fast PCR-based method using our patented DW-ACP primer system for determining the location or orientation of a transgene in transgenic organisms. Since this kit allows the amplification of unknown sequences flanking a known transgene sequence, the insertion position or orientation of a transgene can be determined. Without cloning or library construction of transgenic genomic DNA, whole genomic DNA can be used as a template for direct screening of transgene location or orientation.

Features

• Simple and fast method
• 1-2 kb direct sequencing
• Small amount of starting material required
• Cost-effective
• No false products

5. SpeedUp Deletion/ Insertion/ Isoform Detection

DNA Walking SpeedUp™ Premix Kit provides a simple and fast PCR-based method using our patented DW-ACP primer system for detecting deletion, insertion, or isoform by using whole genomic DNA or total RNA as a starting material. This kit can be used to screen unknown mutations or unknown isoform as well as known mutations or known isoforms. Without a cloning or library construction process, whole genomic DNA or first-strand cDNA can be used as a template for direct screening of deletion, insertion or isoform.

Features

• Simple and fast method
• 1-2 kb direct sequencing
• Small amount of starting material required
• Cost-effective
• No false products