DEG stands for Differentially Expressed Gene and refers to all the genes that are expressed differentially in mRNA level of two or more samples. GeneFishing™ Technology is a new and unique approach capable of identifying only authentic Differentially Expressed Genes (DEGs) in two or more nucleic acid samples. This technology dramatically improves specificity of PCR amplification so that it enables the researchers to find only real PCR products as a result. GeneFishing™ DEG Premix Kits are the first application of Seegene's proprietary technology based on innovative design of the primer (Annealing Control Primer, ACP™, patent pending). No specific skill required! No PAGE gel! No radioactivity! GeneFishing™ Technology is so simple that even the inexperienced in gene discovery can find DEGs showing sufficient resolution on agarose gel within 5 hrs.

Applications & Target Area

• For studying genes which express differentially by each development stage
  Ex. liver regeneration, hepatocyte spheroid formation, spermatogenesis, embryogenesis, stem cell differentiation, developmental biology and cell differentiation, etc.

• For studying disease-related genes (marker, inhibitor) such as cancer
  Ex. congenital heart defects, gastric adenocarcinoma metastasis, pulmanary adenocarcinoma, wound-healing, pre-eclampsia, heart disease, etc.

• For studying genes which express differentially by biological change
  Ex. contaminants, multidrug resistant, signal transduction and growth factor actions, endocrine biology and hormone actions, etc.

• For studying genes which express differentially by biological change or sample treatment (medication or hormone) Ex. heart shock, cold shock, hypoxia, etc.

Fig. 1. Flow chart of differentially expressed genes (DEGs) discovery.
A. Identification of differentially expressed genes by GeneFishing DEG101. Total RNAs from mouse conceptuses at E4.5, E11.5 and E18.5 were used as starting materials for the synthesis of first-strand cDNAs.
B. Confirmation of differentially expression by Northern blot analysis using DEG1, DEG1', or DEG3 as a probe.

Features

• No False positives! (Maximum specificity)
  Only authentic DEGs delivered! No false positives! GeneFishing™ DEG Premix Kits deliver the differentially Expressed Genes (DEGs) which never fail in Northern blot confirmation.

• Guaranteed reproducibility
  GeneFishing™ DEG Premix Kits generate highly reproducible PCR products in every differential display set.

• No specific skill! (Simplicity)
  No specific skill required! No PAGE gel! No radioactivity! GeneFishing™ Technology is so simple that even the inexperienced in gene discovery can find DEGs showing sufficient resolution on agarose gel within 5 hrs.

• Extensive range of PCR product
  Each GeneFishing™ PCR reaction generates long-distance PCR products ranging from 150 bp to 2kb. This wide range of coverage increases the chances to find DEGs and also provides more significant sequence information for the prediction of gene function.

GeneFishing™ Technology is a new and unique approach capable of identifying only authentic Differentially Expressed Genes (DEGs) in two or more nucleic acid samples. This technology dramatically improves specificity of PCR amplification so that it enables the researchers to find only real PCR products as a result. GeneFishing™ DEG Premix Kits are the first application of the Seegene's proprietary technology based on innovative design of the primer (Annealing Control Primer, ACP™, patent pending).

All of the GeneFishing™ DEG Premix Kits (DEG101~106) comprise 20 randomly selected arbitrary ACPs and each DEG Premix Kit works equally for your target samples. If this is your first trial, we recommend the GeneFishing™ DEG101. For further DEG study, we recommend any of the other DEG series (101~106).

Components

20 Arbitrary ACPs (20 reactions)
dT-ACP1 for RT (10 reactions)
dT-ACP2 for PCR (400 reactions)
5' Universal primer
3' Universal primer
Taq polymerase
2mM dNTP
Control cDNAs : Mouse conceptus cDNAs (E4.5 and E18.5)

(* Each items are available for individual purchase.
Please contact us at biogene@biogene.com for details.)

Reagents and Equipment to be Supplied by User

RNase-free H₂O, EtOH (70-100%), Reverse transcriptase, RNase inhibitor,
Thermal cycler, Micro-centrifuge.

Storage Conditions

This product is shipped at ambient temperature. Store the template below -20°C for
long term storage. Avoid multiple freeze-thaw cycles.