Introduction

GeneXp™ Kit - the ideal tool for gene expression profiling.

GeneXP™ kits is Seegene’s original DSO™ -based multiplex
RT-PCR system.

• Only a single reaction in necessary to amplify multiple genes of interest.
• One reaction is sufficient to amplify multiple genes simultaneously in a single reaction.

GeneFishing™ DEG Dicovery kit uses randomly designed arbitrary primers to discover DEGs (differentially expressed genes) in the samples, whereas the GeneXp kit uses target specific primers for target gene profiling.

Multiplex PCRs capacity to simultaneously quantify miltiple mRNA species from a single of RNA allows comparative analysis of different mRNA species within samples. Variations in RNA isolation, initial quantification errors or tube-to-tube variations in RT and PCR can be compensated by including a known housekeeping gene. DSO™ primers can simultaneously detect multiple target genes without creating any false products.

Advantages & Features

Comparison with other methods
To date, gene expression patterns are often analysed by microarray methods that involve a DNA chip or by PCR-based methods.

Microarray

• Microarray analysis has the advantage over the other methods in that it permits high throughput screening at one time. However, it has many potential pitfalls, such as a high false-positive rate and low sensitivity. Moreover, the number of samples that can be compared is restricted, and it is difficult to detect subtle differences between the samples.

• Experiments need to be repeated due to the low reproducibility.
• Hybridisation method is comparably lower in sensitivity than PCR method.
• Additional experiments (Northern blot, Real-time PCR, RT-PCR, etc.) are required for validation.
• All the clones with positive signal need to be checked, hence requiring more cost and time for confirmation steps.

  GeneXp Kit

• Systematic Analysis of Gene Family Expression.
• High Specificity & Sensitivity using by DSO™-based multiplex RT-PCR.
• No more confirmation step required.

Applications

• Comparison of normal vs. diseased tissue
  EX. normal vs. tumor, non-infected vs. infected, wild type vs. knock-out
• Time courses
  EX. developmental stage, environmental change
• Test of influence of Chemical compounds
  EX. Drug, chemical, hormone, post-clinical treatments
• Microarray data validation, etc.
  

For further information on this new application please see the following:

• Seegene Diagnostic Products (PDF - 732Kb)