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Respiratory Viruses Detection Kit |
Examples
Comparison with other respiratory detection kits that are
using multiplex PCR
| Multiplex PCR based Kit |
Company |
Virus |
Total No. |
| Respiratory Virus Detection Kit |
Seegene |
Adenovirus, Metapneumovirus Coronavirus 229E/NL63, Parainfluenza-1,
2, 3 Influenza A, Influenza B Respiratory syncytial virus A,
Respiratory syncytial virus B, Rhinovirus, Coronavirus OC4 V |
12 type |
| M PCR Kit |
M Company |
Adenovirus, Coronavirus, Influenza A, Influenza B , Parainfluenza-1,
2, 3, Rhinovirus, RSV |
9 type |
| H Kit |
P Company |
Metapneumovirus, Influenza A Influenza B, RSV, Parainfluenza-1,
- 2, -3 |
7 type |
A Coverage for 12 respiratory viruses
In the case of other company’s respiratory virus detection
kits using the multiplex PCR method, the maximum number of analyses
available is 7 types of virus. In the case of the detection methods
using antibodies, the number of analyses available is decreased
further.
Seegene’s RV detection kit is designed to detect 12 types
of respiratory virus, some of which are not detectable by other
commercial virus detection kits.
Figure 1: Multiplex PCR result of 12 viruses using Seegene's
RV kit
Viral RNA from a viral culture soup was purified and then a reverse
transcription reaction was performed. cDNA was used as a template
in mono PCR and multiplex PCR which was conducted with the SRVD
primer mix. This figure is a confirmed result on 2% agarose gel.
High Sensitivity
Figure 2: An example of sensitivity. The minor band as
well as major bands were confirmed as low expressed virus, not a
false signal.
In general, the sensitivity of multiplex PCR is much lower than
that of mono PCR due to primer interference. The low sensitivity
of multiplex PCR sometimes results in a false negative. However,
Seegene’s DSO technology enhances the sensitivity of the multiplex
PCR, resulting in a higher sensitivity of Seegene’s respiratory
virus detection kit compared to other company’s multiplex
respiratory virus detection kits.
This kit shows excellent results in virus infection analysis at
an early stage because detection is available on a minimum amount
of virus in the sample.
High Specificity
Figure 3: An example of specificity.
Because this product uses DSO technology to muliplex PCR, a non-specific
band does not appear and it is possible to detect only the virus
that exists in the patient’s sample.
Therefore, both viruses that can possibly generate disease, as
well as viruses that become a major cause of disease, can be precisely
detected.
Result of respiratory virus detection analysis
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