Enhanced SNP & Indel Analysis
of Illumina® Genome Analyzer data in latest release
of NextGENe software suite
July 11, 2008, State College PA,
announced the release of the latest version of its NextGENe
software which includes improved SNP and Indel capabilities
over prior versions of the software. A recent study conducted
in collaboration with a leading Midwestern medical research
institution on mitochondrial DNA samples from several individuals
were sequenced on the Illumina® Genome Sequencing system
and analyzed with both the NextGENe and in-house developed
software. NextGENe results were extraordinary with less
than .5% false positives and less than no false negatives.
"Crucial to accurate analysis of short
fragment sequencing, states Jonathan Liu, VP development,
is correct assembly and lignment. In NextGENe we have incorporated
a very unique condensation tool (patent applied for) which
statistically polishes the data, removing chemistry and
instrument related errors, followed by raw data sorting
by a 12 bp anchor sequence and the sequence shoulders and
finallyassembly by further condensation cycles. The assembled
sample data is then compared to a gen bank or other reference
with the software automatically displaying both SNP and
Indels".
In addition to Illumina Genome Analyzer
data, user of the Roche 454 and Applied BioSystems SOLiD™
system can benefit from the unique capabilities of NextGENe
software. NextGENe has been designed to efficiently operate
on low-cost 64 bit Windows computers, features a biologist-friendly
Windows interface, significantly reducing the need for bioinformatics
expensitures. NextGENe analysis modules include SNP/Indel
detection, de novo and target assembly, Digital Gene Expression
analysis, Transcriptome, ChipSeq, miRNA discovery and quantification.
Interested parties may request a free 30-day evaluation
or schedule.
SoftGenetics specializes in the development
of genetic analysis tools for both research and diagnostic
applications. Hallmarks of SoftGenetics software tools are
advanced technologies, providing exceptional accuracy, and
sensitivity in easy-to use Windows® user interface.
