Avian Influenza Virus (AIV)
Direct detection of inactivated AIV of the current UK outbreak strain, spiked into claocal swab at 6-100 virions/rxn. LLOD is <1,000 virions/ml of eluate
Detects >99.9% of Known AIV Strains Worldwide
The AIV assay is built on complete global sequence coverage over 7 years, using a comprehensive primer set and single universal probe.
- Sample type: Cloacal or oropharyngeal swabs
- Preparation: Elute into 1 ml water; add 16% v/v to reaction – no extraction
- LOD: <1,000 virions/ml
- Time to result: ~30 minutes
- Cross-reactivity: None observed across broad avian pathogen panel
Use for farm-side screening, import/export testing, or wildlife surveillance. H5 and H7 subtype-specific assays are in development.
Performance data generated through an Innovate UK funded research project, involving the Animal & Plant Health Agency (APHA), demonstrate the rapid molecular detection capability of the CENOS platform under defined study conditions. The participating institutions do not endorse the product.
Direct detection of inactivated AIV of the current UK outbreak strain, spiked into claocal swab at 6-100 virions/rxn. LLOD is <1,000 virions/ml of eluate.
Showing a standard curve for the virus responsible for the current UK outbreak, run from unextracted viral antigen using the CENOS XF AIV assay.
Showing a standard curve for a Chinese H3N3 variant that caused an outbreak in 2024, run from unextracted viral antigen using the CENOS XF AIV assay.
Demonstrating that viral growth medium added at 11% was minimally inhibitory to the CENOS Xtraction Free reagents.
Data for showing >60 different CP and 40 OP swabs, showing that Ct can be delayed by up to 0.9 cycles, but assay LLOD is not impacted.
Data shows detection from pooled swabs, where 5 cloacal swabs were pooled and tested. It is also possible to concurrently test the OP and CP swab in the same CENOS XF reaction.
Cloacal swab added at 11% and spiked with AIV IVT RNA down to 6 targets/rxn – equivalent to <1,000 virions/swab, or <0.2 targets/ul rxn.
The CENOS XF AIV assay was specific and did not detect high titres of other UK endemic viral pathogens.