Mutation Surveyor^®

A Unique tool for DNA Variant Analysis from Sanger Sequencing Traces

Mutation Surveyor^® software is a powerful and accurate DNA Sequencing analysis tool for Sanger Sequencing files generated by the following electrophoresis systems:

Accurate, rapid genotyping and chimerism analysis with documented time savings of up to 85%
Beckman CEQ
Promega Spectrum Compact CE System

Mutation Surveyor software includes patented anti-correlation technology, which physically compares sample sequence traces to a reference trace, providing accuracy up to 99.5% with Phred 20 bi-directional sequences and a sensitivity of 5% of the primary peak.

Capable of performing variant analysis of up to 2000 Sanger sequencing files (.ab1, .RSD, .ESD & .scf) in 15 minutes, Mutation Surveyor delivers excellent accuracy, sensitivity, and low false-positive and false-negative rates in the analysis of DNA variants, including:

Single nucleotide polymorphisms (SNPs)
Homozygous insertions and deletions (indels)
Deconvolution and detection of heterozygous indels
Somatic mutations in direct sequencing

The Mutation Surveyor package includes patented anti-correlation technology (U.S. Patent 8,086,410), which physically compares sample sequence traces to a reference trace, providing accuracy up to 99.5% with Phred 20 bi-directional sequences and a sensitivity of 5% of the primary peak.

Along with increased accuracy and sensitivity, Mutation Surveyor software features many tools:

Custom reporting
Automated download of annotated GenBank reference files
DNA methylation detection
Mitochondrial DNA sequence analysis
Allele quantification
Variant Knowledge Database

Mutation Surveyor software includes features designed to enhance and optimize the process of variant discovery in Sanger sequencing traces. One such feature is the variant knowledge database which allows users to track variants by chromosomal position, indicate false-positives and other artefacts, and query mutation annotation information from popular variant databases such as dbSNP, dbNSFP, ClinVar, gnomAD, and COSMIC.

Also included in Mutation Surveyor software is an intuitive user management system, which enables administrators to set up password-protected user names, control access rights, and generate a user audit trail. Access rights for each user are determined by the administrator, providing assurance that unauthorized individuals do not accidentally alter a project or change the analysis parameters established by the laboratory. User management also provides the user ID in the header of the final reports.